| Oral Toxicity Test |
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Eurofins | Product Safety LabsMarch 30, 2007
Enclosed please find the following final reports for Artic Fire- Freeze™: Study #5482 - Acute Oral Toxicity Study in RatsBy this letter we are forwarding to you our testing and historical results of our Acute Oral Toxicity Limit testing conducted on your Arctic Fire-Freeze™ fire extinguishing agent. The conclusion of the test showed that when a dosage of 5,000 mglkg of bodyweight was administered, each animal survived, gained weight and appeared active healthy. There were no signs of gross toxicity, adverse pharmacologic effects of abnormal behavior. The product meets the national standard for a non-toxic substance. Sincerely, EUROFINS | PRODUCT SAFETY LABS
Lisa Buber ACUTE ORAL TOXICITY LIMIT TEST
PURPOSE:
To provide information on health hazards likely to arise from a single exposure to Arctic ire-~reeze~~ PROCEDURE:A group of Sprague-Dawley derived, albino rats was received from Ace Animals, Inc., Boyertown, PA. The animals were singly housed in suspended stainless steel caging with mesh floors. Litter paper was placed beneath the cages and was changed at least three times per week. The animal room was temperature controlled and had a 12-hour light/dark cycle. The animals were fed Purina Rodent Chow #5012 and filtered tap water was supplied ad libitum by automatic watering system.
Following acclimation to the laboratory, a group of animals was fasted for approximately 22 hours by removing feed tiom their cages. After the fasting period, ten rats (five male and five female) were selected for test based on health and initial bodyweights. Individual doses were calculated based on these bodyweights, taking inlo account the spucifTc gravity (determined by EPSL) oflhe test material. Each animal received 5,000 mg/kg of the test material by intubation using a stainless steel ball-tipped gavage needle attached to an appropriate syringe. After administration, each animal was returned to its designated cage. Feed was replaced approximately three hours after dosing. RESULTS:All animals survived, gained weight and appeared active and healthy. There were no signs of gross toxicity, adverse pharmacologic effects or abnormal behavior.
The Single Dose Acute Oral LD50 of Arctic Fire Freeze™ is greater than 5,000 mg/kg of bodyweight when Arctic Fire-Freeze™
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| Animal Number | Sex | Weight - Initial |
Weight - Day 14 |
Dose* mL |
| 9304 | M | 196 | 332 | 0.85 |
| 9305 | M | 193 | 355 | 0.84 |
| 9306 | M | 186 | 337 | 0.81 |
| 9307 | M | 193 | 344 | 0.84 |
| 9308 | M | 188 | 328 | 0.82 |
| 9309 | F | 158 | 229 | 0.69 |
| 9310 | F | 182 | 232 | 0.79 |
| 9311 | F | 161 | 227 | 0.70 |
| 9312 | F | 163 | 221 | 0.71 |
| 9313 | F | 162 | 231 | 0.71 |
*Administered as received. Specific gravity - 1.148 g/mL
Product Safety Laboratories
Table 2
INDIVIDUAL CAGE-SIDE OBSERVATION
| Animal Number | Finding | Day of Occurence |
| MALES | ||
| 9304-9308 | Active and Healthy | 0-14 |
| FEMALES | ||
| 9309-9313 | Active and Healthy |
0-14 |
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